Figure 7.

HMP-S7 induced patient-derived leukemic cell death ex vivo. Bone-marrow-derived lymphoblasts were collected from three leukemic patients and were processed as described in “Materials and Methods” section. (a) Demographic data of three leukemic patients. Patient-derived lymphoblasts were treated with two doses of HMP-S7 or with BMP-S6 (a positive control from previous experiments); (b) flow cytometric analysis with annexin V/PI co-staining at 72-h post-treatment. Patient-derived leukemic cells were treated with 200 and 400 µM HMP-S7, while 200 µM BMP-S6 was included for comparison; (c) % cell death including the upper left, upper right, and lower right quadrants of flow cytometric data (n = 4 replicates per condition). Abbreviations: B-ALL, B-cell acute lymphoblastic leukemia; F, female; M, male; MRD, minimal residual disease; NCI, National Cancer Institute; WBC, white blood cells.