Figure 2.

Interaction between H₄R with TSPAN4 and CD63 as measured by MYTH liquid growth assay. (A) Co-expression of the H₄R-Cub-LexA-VP16 and NubG-TSPAN4 constructs is evidenced by growth in media without leucine and tryptophane (black trace, selecting for diploids). Two-hybrid interaction and subsequent expression of the HIS3 reporter gene results in growth on media additionally depleted for histidine (green trace, selecting for a two-hybrid interaction). (B) Modulation histidine-independent growth of the H₄R-Cub-LexA-VP16 and NubG-TSPAN4 expression diploid by vehicle (blue trace) or 10 µM histamine (red trace). (C) Interaction between H₄R-Cub-LexA-VP16 and NubG-CD63 as describe in (A). (D) Modulation of the interaction between H₄R (bait) and CD63 (prey) as described in (B). Data are displayed as mean ± SEM from 3 independent growth traces.