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COUP-TFI activates the mdm2 p53 response sequence. HeLa cells were transfected by electroporation with 4 μg of the indicated reporters that contain different fragments of the first intron of the mdm2 gene and 4 μg of a pRSV control vector (−COUPTFI) or pRSV-COUP-TFI (+COUPTFI). Following transfection, cells were incubated in hormone-depleted medium for 48 h prior to determination of CAT activity.
