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. 2021 Aug 8;13(16):3997. doi: 10.3390/cancers13163997

Figure 1.

Figure 1

Generation of human hepatocellular carcinoma (HCC) organoid. (a) Bright-field image of the protocol for the generation of human HCC organoid. Morphology of human HCC organoid in the Ibidi culture-insert 2 well system at day 6. After 6 days of cultivation, the human HCC organoid was generated. Red arrows indicate single mini HCC organoid. The red dotted line indicates the area of each mini HCC organoid. The black dotted line indicates the rectangular tissue block. The scale bar represents 500 μm. (b) Live imaging of KO-EC with Huh7-Luci and iPSC-MC in the Ibidi culture-insert 2 well system at day 1, 2, and 6. The scale bar represents 200 μm. (c) ELISA of ALB secretion in HCC organoid at day 2, 4, and 6. One-way ANOVA followed by Tukey’s multiple comparisons test (n = 6/group). (d) Luciferase measurement of Huh7-Luci in HCC organoids generated by different composition ratio of Huh7-Luci, iPSC-EC, and iPSC-MC. One-way ANOVA followed by Tukey’s multiple comparisons test (n = 6/group). ** p < 0.01, **** p < 0.0001.