Percentage of viability at 24 h postillumination for (a) HepG2; (b) U87. NT, nontreated; 5-ALA, Photosensitizer only; Ill1, illumination in 1 fraction; Ill2, illumination in 2 fractions; Ill3, illumination in 3 fractions; Ill4, illumination in 4 fractions; PDT1, illumination in the presence of 5-ALA in 1 fraction; PDT2, illumination in the presence of 5-ALA in 2 fractions; PDT3, illumination in the presence of 5-ALA in 3 fractions; PDT4, illumination in the presence of 5-ALA in 4 fractions. Results are presented as box plots, expressed in % of the NT. ANCOVA statistical test was performed, all quoted p-values are two-sided, with p ≤ 0.0001 (***) considered statistically significant, and p ≥ 0.05 considered not significant (NS).