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. 2021 Aug 17;13(16):4143. doi: 10.3390/cancers13164143

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Influence of medium composition on SPION-loading of T cells. (A) Experimental setup. (B) Determination of the cell count by flow cytometry. (C) The viability of the cells was determined by AxV-FITC and PI staining in flow cytometry. AxV-FITC-/PI- cells were considered viable, AxV-FITC+/PI- apoptotic, and PI+ cells necrotic. (D) Estimation of cellular nanoparticle loading by analysis of the side scatter increase of viable cells by flow cytometry. (E) Cellular iron content [pg Fe/cell] was determined by atomic emission spectroscopy of cell lysates. (B–E) Experiments were performed in triplicates of T cells from 3–5 donors. Shown are the mean values with standard deviations. Significances were calculated with a 2-way ANOVA test with a Tukey post hoc test; * p ≤ 0.05; *** p ≤ 0.001. ctrl: control; SSC: side scatter; med: medium; ns: not significant; nec: necrotic; apo: apoptotic; viab: viable.