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. 2021 Aug 15;13(16):4106. doi: 10.3390/cancers13164106

Figure 2.

Figure 2

Cell numbers were determined in (A) H1975 and (B) H1650 lung cancer cells after transfection of 50 nM scrambled control (Ctrl) and ANXA1 (SiANXA1) siRNA with or without 10 nM Osimertinib for 72 h. Cell numbers were normalized to control siRNA transfected groups. The experiments were performed in triplicate. Anchorage-dependent colony formation assay in (C) H1975 and (D) H1650 lung cancer cells. After transfection of 50 nM scrambled control (Ctrl) and ANXA1 (SiANXA1) siRNA, cells were treated with or without 10 nM Osimertinib for 14 days. Anchorage-independent 3D culture in (E) H1975 and (F) H1650 lung cancer cells after transfection of 50 nM scrambled control (Ctrl) and ANXA1 (SiANXA1) siRNA with or without 10 nM Osimertinib for 10 days. Relative colony formation is represented by normalization of the colony number to the control group and shown as bar ± standard deviation in triplicate experiments. “ns” denotes nonspecific. “*” denotes p < 0.05; “**” denotes p < 0.01; “***” denotes p < 0.001.