Skip to main content
. 2021 Aug 15;13(16):4106. doi: 10.3390/cancers13164106

Figure 6.

Figure 6

(A). Western blot analysis of ANXA1-GFP in H1975 and H1650 lung cancer cells transfected with pCMV6-AC-GFP (EV) and pCMV6-ANXA1-GFP (ANXA1). Cell numbers were determined in (B) H1975 and H1650 lung cancer cells treated with or without 10 (H1975) or 20 nM (H1650) of Osimertinib for 72 h. Cell numbers were normalized to the EV group. The experiments were performed in triplicate. (C) RT-PCR of the ectopic EGFR in H1975 lung cancer cells transfected with L858R + C797S EGFR mutations (H1975C797S). (D) Cell numbers were determined in H1975 and H1975C797S lung cancer cells treated with or without 10 nM Osimertinib for 72 h. Cell numbers were normalized to the H1975 group. The experiments were performed in triplicate. (E) H1975C797S lung cancer cells after transfection of 50 nM scrambled control (Ctrl) and ANXA1 (SiANXA1) siRNA with or without 10 nM Osimertinib for 72 h. Cell numbers were normalized to control siRNA transfected groups. The experiments were performed in triplicate. “ns” denotes nonspecific. “*” denotes p < 0.05; “**” denotes p < 0.01; “***” denotes p < 0.001. Uncropped western blot figures were included in Supplementary Materials.