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. 2021 Jul 23;10(8):1863. doi: 10.3390/cells10081863

Figure 6.

Figure 6

Altered MD in reperfused young and aged hepatocytes. (A) IB analysis of proteins related to MD after I/R in hepatocytes from different ages (n = 3). Changes in individual proteins were expressed relative to their levels in young hepatocytes at normoxia. (B) Determination of L- and S-OPA expression in reperfused aged hepatocytes before and after CAST overexpression (n = 3). Changes in S-OPA1 were expressed relative to its level at normoxia without CAST overexpression. (C) Confocal microscopy of TMRM-labelled mitochondria with and without CAST overexpression. Bottom panels represent enlarged images of the square inset in top panels. * p < 0.05, ** p < 0.01 and *** p < 0.001.