FIG. 4.

Transcriptional activity of purified Ppr1p. In vitro transcription reactions contained 50 nM purified Ppr1p where indicated and were supplemented with the compounds indicated, each at a final concentration of 1 mM, except for aspartic acid (1.6 mM) and carbamoyl aspartic acid (0.8 mM). Transcription products from the template shown at the top were analyzed by primer extension. Positions of the primer and the E4 extension products are indicated.