FIG. 5.

Concentration effects of Ppr1p, DHO, and OA. (A) In vitro transcription reactions contained pG₅E4 (lanes 1 to 4) or pPPR₇E4 (lanes 5 to 16) as template DNA. Reactions contained Gal4p (amino acids 1 to 93 fused to 768 to 881) or Ppr1p at the levels indicated. DHO was added to the reactions as indicated. (B and C) In vitro transcription reactions were performed with pPPR₇E4 as the template DNA. Reactions contained Ppr1p and either DHO or OA at the concentrations indicated. Positions of the primer and the E4 extension products in each case are indicated.