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The C-terminal region of Ppr1p is responsive to OA-induced stimulation of transcriptional activity. Transcription reactions contained plasmid pG5E4 and Gal4p(1-93)–Ppr1p(770-904) at either 250 nM (lanes 2 to 5) or 450 nM (lanes 6 to 9). The reactions were supplemented with OA at the concentrations indicated. Positions of the primer and the E4 extension products are indicated.
