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. 2021 Jul 29;2(5):zqab036. doi: 10.1093/function/zqab036

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© The Author(s) 2021. Published by Oxford University Press on behalf of American Physiological Society.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Store-operated Ca²⁺ entry does not generate a detectable cAMP rise near adjacent AKAP79. (A) Cartoon depicts the principle underlying FRET between CFP and YFP for plasma-membrane targeted AKAP79-CUTie. CNBD denotes cyclic nucleotide binding domain. (B) Stimulation with thapsigargin failed to generate a FRET signal from AKAP79-CUTie whereas a robust response was seen following stimulation with forskolin and IBMX. (C) Aggregate data from 6 cells from 3 independent experiments are shown. (D) Proximity ligation assay shows increased association of AKAP79-CUTie with Orai1-myc after stimulation in HEK293 cells. Images compare proximity of AKAP79-CUTie and Orai1-myc in HEK293 cells at rest and then after 15 min exposure to thapsigargin. Nuclei were stained with DAPI. (E) Aggregate data from experiments as in Panel B are compared (>12 cells per bar).