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. 2021 Jul 29;2(5):zqab036. doi: 10.1093/function/zqab036

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© The Author(s) 2021. Published by Oxford University Press on behalf of American Physiological Society.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Identifying the forskolin-sensitive adenylyl cyclase in HEK293 cells. (A) Stimulation with forskolin evokes an increase in cAMP, measured with the H187 FRET probe. (B) Aggregate data are compared (each bar is the mean of between 8 and 11 cells). Rest denotes the resting condition prior to forskolin treatment. (C) Activation of β1 adrenoceptors with 100 μm isoproterenol produces a robust increase in cAMP. (D) Aggregate data from 7–10 cells are compared. (E) Forskolin-stimulated protein kinase A activity is almost completely suppressed in AC3/AC6 double knockout (DKO) cells.