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Evolution of the UV-vis absorption spectrum of a test sample (‘CA_20.5’) containing chlorophyll a diluted in refined oil, after a first (at time = 0′) and a second (at time = 117′) acidification, as described in the text. Spectra were recorded during time, from 0 to 166 min, following the complete conversion from chlorophyll a to pheophytin a. The optical path was 0.5 cm.
