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. 2021 Aug 10;10(8):2046. doi: 10.3390/cells10082046

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Measurements of superoxide production in primary hippocampal neurons. Basal MitoSOX fluorescence. WT, yellow, and B5.152H, purple, hippocampal neurons loaded with 200 nM MitoSOX™. The result is expressed as the average fluorescence for 8 WT and 6 B6.152H coverslips, respectively, with multiple neurons, derived from at least three independent preparations. For p ≤ 0.05, the differences between the two genotypes were not statistically significant. Columns represent mean value plus/minus SD.