Table 2.
The primers used in this work
| Name | Primer sequence (5´ → 3´) | Amplification product | Length (bp) |
|---|---|---|---|
| Hyg-F | GTCGACGTTAACTGATATTG | hygromycin resistance gene (hyg) | 1389 |
| Hyg-R | TTTGCCCTCGGACGAGTGCT | ||
| Ku70-L-F | GGGGTACCGAGCTCGAGGCCAAACAGGCAG | ku70 | 1800 |
| Ku70-R-R | CCCAAGCTT TCTAGATAACTGTACATCGCCT | ||
| PglaA-F | CGATAGATCTGGATCCCTGCTCTCTCTCTGCTCT | glaA promoter | 1493 |
| PglaA-R | CAACAACATGTGAGGAGGTGAACGAA | ||
| HGT1-F | CACCTCCTCACATGTTGTTGATTGGCAACAT | HGT1 | 1819 |
| HGT1-R | CGACTCTAGAGGATCCTTATGCTGTGGCCTCCTGGG | ||
| Paox1-F | CGATAGATCTGGATCCGACACCGAGCACATGA | aox1 promoter | 1404 |
| Paox1-R | GTTGCCAATCAACATCGGGTATAGAACCACAG | ||
| GKqrt-F | CCGCAATGAGAAGAATGG | qPCR for glucokinase | |
| GKqrt-R | CATCGGGAATGTTGAAGC | ||
| CSqrt-F | ACGGCAAGACCAAGAACC | qPCR for citrate synthase | |
| CSqrt-R | CACGGGAAACACCGAAGA | ||
| HGT1qrt-F | CGGTATGCTCGTTGTTGG | qPCR for HGT1 | |
| HGT1qrt-R | GGCGAAAGTTCACTGATGTA | ||
| 18Sqrt-F | TCGCTACTACCGATTGAA | qPCR for 18S rRNA | |
| 18Sqrt-R | CACCTACGGAAACCTTGT |
The EcoR I, Kpn I, Pst I and Hind III sites are underlined