TABLE 2.
Growth properties of PC4-transfected 9113 ras-Transformed PA-1 cellsa
| Cell line | Cell countb | % Anchorage-independent growthc |
|---|---|---|
| 9113-zeo-1 | 12 × 106 | 8.5 |
| 9113-zeo-2 | 15 × 106 | 11 |
| 9113-zeo-PC4-1 | 1.5 × 106 | 0.1 |
| 9113-zeo-PC4-2 | 1.5 × 106 | 0.06 |
| 9113-zeo-PC4-4 | 0.9 × 106 | 0.13 |
a
Comparison of the growth properties of PA-1 9113 ras-transformed cells stably transfected with the vector control (zeo-1 or zeo-2) or the PC4 expression vector (PC4-1, PC4-2, or PC4-4).
b
Averages of counts from duplicate dishes. Cells (105) were seeded in duplicate in 60-mm-diameter culture dishes, trypsinized after 8 days of growth, and counted with the aid of a hemocytometer.
c
Cells (104) were seeded in 0.35% agarose in complete medium (modified Eagle’s medium with 10% fetal bovine serum) and overlaid on a bed of 0.7% agarose in complete medium. The numbers of colonies were determined after 14 days.