Table 2.
List of studies highlighting applications of microsatellites in the characterization of olive genetic resources.
| Objective | Cultivars or Accessions |
Region | No. of SSRs | Ho | He | Na | PIC | Reference | Key Remarks |
|---|---|---|---|---|---|---|---|---|---|
| Characterization and Identification of Olive Cultivars | 19 | Slovenia, Italy, France, Spain | 14 (DCA-1,3,4,5,7,8,9,10, 11, 13, 14, 15, 16, 17) | 0.263–1.000 | NA | 3–12 | NA | [60] | Identification key of 19 olive varieties |
| Characterization and Identification of Olive Cultivars | 87 | Iran | 16 (DCA 18, 17, 16, 15, 14, 13, 11, 10, GAPU101, 103A, 89, 71B, 72, 90) | NA | NA | NA | 0.620–0.950 | [92] | Intra-cultivar variation, cultivar denominations and origin investigated using SSRs |
| Characterization of Autochthonous Olives | 44 | Croatia | 16 (UDO-08, 12, 19, 24, 28, 31, 39, 43, DCA3, 8, 9, 10, 14, 16, EMO2, 3) | 0.273–0.932 | 0.499–0.910 | 5–20 | NA | [93] | SSR-based varietal discrimination achieved |
| Germplasm Characterization | 154 | Tuscany | 12 (UDO-04, 06, 09, 11, 12, 17, 19, 24, 27, 31, SIU06, 08) | 0.278–0.722 | 0.428–0.855 | 3–10 | NA | [94] | Homonyms and synonyms detected |
| Database Development and Cultivar Identification | 17 | Mediterranean Basin | 08 (DCA3, 4, 8, 9, 11, 13, 14, 15) | NA | NA | 3–12 | NA | [57] | Standardization of SSR set for olive cultivar studies |
| Characterization and Identification of Olive Cultivars | 18 | Bologna, Italy | 17 (DCA3,4,5,7,9,13,14,15,16,17,18, EMO90, GAPU59,101,103, UDO-24, 43) | 0.500–1.000 | 0.431–0.841 | 4–10 | NA | [95] | Synonyms identified and diversity in germplasm revealed |
| Characterization of Olive Cultivars | 20 | Tunisia | 10 (GAPU59, 71A, 71B, 103A, UDO-03, 12, 28, 39, DCA9, 18) | 0.300–0.950 | 0.562–0.801 | 3–6 | NA | [96] | Cultivars broadly grouped by their end use and phenotypes |
| Characterization of Olive Cultivars | 38 | Southern Marmara region, Turkey | 10 (GAPU103A, 101, UDO-06, 07, 09, 11, 12, 14, 15, 35) | NA | NA | 2–5 | NA | [97] | Cultivar Gemlik revealed as major olive cultivar in the region |
| Characterization of Olive Cultivars | 51 | Iran | 13 (DCA3,9,16,18,11,15, UDO-43, 11, 19, 24, GAPU59, 71B, 101) | 0.000–1.000 | 0.000–0.800 | 1–8 | 0.000–0.750 | [98] | Synonyms, homonyms and intra-cultivar polymorphisms detected |
| Characterization and Diversity Analysis | 47 | Southern Italy | 6 (DCA3, 4, 16, 17, UDO-31, GAPU 47) | 0.700–0.890 | NA | 11–17 | 0.830–0.870 | [99] | Genetic distinctness of accessions from Campania region established |
| Characterization of Olive cultivars | 10 | Turkey | 7 (DCA-4, 9, 11, 16, 17, GAPU-89, UDO-14) | NA | NA | 3–6 | NA | [100] | Misnamings among cultivars identified |
| Cultivar Identification Using SSR | 53 | Australia | 7 (9, 3, 16, 18, 5, EMO90, 30) | 0.490–0.980 | 0.480–0.840 | 7–12 | 0.467–0.813 | [101] | Samples grouped into distinct genotypes |
| Characterization of Olive Germplasm | 561 | Marrakech (OWGB collection) | 17 (DCA1, 3, 4, 5, 8, 9, 11, 14, 15, 18, UDO-36, GAPU59, 71A, 71B, EMO03, 90,PA(ATT)2*) | 0.490–0.928 | 0.454–0.876 | 5–32 | 0.403–0.864 | [102] | Construction of two core collections |
| Cultivar Characterization and Diversity Analysis | 26 | Algeria | 11 (DCA9, 18, GAPU59, 71A, 71B, 101, 103A, UDO-12, 43, 28, 39) | 0.135–0.889 | 0.070–0.510 | 6–21 | NA | [103] | SSR genotyping allowed unambiguous identification of all the cultivars |
| Cultivar characterization and Diversity Analysis | 39 | Colombia | 10 (DCA3, 5, 9, 16, 17, 18, UDO-43, GAPU101, 103A, EMO90) | 0.250–1.000 | 0.312–0.909 | 3–15 | 0.282–0.876 | [104] | Synonyms, homonyms identified and 19 genetic profiles discriminated |
(Na) Average number of alleles per locus. (Ho) Observed heterozygosity. (He) Expected heterozygosity or gene diversity. (PIC) Polymorphic information content.