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. 2021 Aug 5;22(16):8405. doi: 10.3390/ijms22168405

Figure 2.

Figure 2

SH003 in combination with docetaxel induces apoptosis in H460 and A549 cells. (A) To detect apoptosis induced by SH003 and docetaxel, H460 and A549 cells were double stained with annexin V-FITC and 7-AAD for 24 h. (B) Expression levels of poly (ADP-ribose) polymerase (PARP), cleaved PARP, cleaved caspase-3, and cleaved caspase-7 were determined by Western blotting in H460 and A549 cells treated with SH003 and docetaxel for 24 h. Statistical significance was determined using the one-way ANOVA with Tukey’s test comparing the control; * p < 0.05, ** p < 0.01, *** p < 0.001, and comparing the combination; # p < 0.05, ## p < 0.01, ### p < 0.001. (C) H460 cells were pre-treated with 20 μM Z-VAD-FMK, a pan-caspase inhibitor, followed by their combination for 48 h. Cell viability was analyzed by WST-1 assay. Data are presented as the mean ± standard deviation (n = 3). Statistical significance was determined using the one-way ANOVA with Newman–Keuls multiple comparison test comparing control and combination treatment; * p < 0.05 and *** p < 0.001.