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. 2021 Aug 5;22(16):8417. doi: 10.3390/ijms22168417

Figure 3.

Figure 3

The N-methyl-D-aspartate receptor (NMDAR)/α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) ratio was significantly reduced in the CTX-treated animals, with no effect on NMDAR-mediated current kinetics. (a) AMPAR-mediated evoked excitatory postsynaptic currents (eEPSCs) recorded at −80 mV in the presence of GABAaR and GABAbR antagonists bicuculline (10 µM) and CGP 55845 (5 µM). (b) Complete block of the AMPAR-mediated current by bath-applied DNQX (20 µM). (c) NMDAR-mediated eEPSCs recorded in the same neuron in the presence of DNQX at −20 mV with the same stimulus current amplitude. (d) Average NMDAR-mediated currents normalized by the AMPAR-mediated eEPSC amplitude for each group. The transparent areas represent the standard error of the mean for each trace. (e) NMDA/AMPA ratios measured as the peak NMDAR-mediated current amplitude (for the last EPSC) divided by the peak AMPAR-mediated current amplitude. The asterisk represents a significant difference according to the Student’s t-test (p < 0.01). (f) Average NMDAR-mediated peak eEPSC amplitudes normalized by the first EPSC amplitude for each recorded cell. (g) Decay τ measured at the 80–20% amplitude of the last NMDAR-mediated EPSC in the train using a monoexponential approximation. ** p < 0.01, a significant difference versus the control group according to the Student’s t-test.