Figure 4.

Increased exon skipping caused by Anti-N1 treatment. (A). The sequence context of 20 candidate exons exhibiting greatly increased skipping upon Anti-N1 treatment. Table contents and annotation are the same as in Figure 3A. (B). Enriched sequence motifs identified by MEME. Left panels: the two most enriched motifs within 50 nucleotides upstream of the 3′ss of affected exons, both corresponding to the polypyrimidine tract and 3′ss sequence. Middle panel: the most enriched motif within the affected exons. Right panels: two motifs enriched downstream of the affected exons. The top motif corresponds to the consensus 5′ss, and the bottom one corresponds to a C-rich motif. n = 100 sequences. (C). Genomic views of several candidate exons whose skipping is increased upon Anti-N1 treatment. For each genomic region an exon whose skipping is increased by Anti-N1 treatment is specified with a red arrow. Alternative 5′ss and 3′ss are indicated with red extensions to the original exon. The rest of the labeling and coloring is the same as in Figure 3C.