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Alizarin red staining for samples cultured in osteogenic differentiation medium with Lyosecretome or without (CTR) for 14, 28, and 56 days. Multifactor ANOVA (time and treatment, mean values ± LSD, n = 3) was used. Both time and treatment were significant (p < 0.0001 and p = 0.0142, respectively). Different letters indicate a significant difference between the means (p < 0.05), while the same letters indicate no significant difference between the means (p > 0.05).
