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. 2021 Aug 6;22(16):8458. doi: 10.3390/ijms22168458

Figure 2.

Figure 2

Regeneration of wild-type and polQ mutant rice plants after transformation by an Agrobacterium strain lacking or harboring a wild-type rice PolQ gene in the T-DNA. Transgenic calli (wild-type Nipponbare or three different polQ mutant lines (see [106]) were selected on medium containing 35 mg/L geneticin (G418) and 25 mg/L meropenem for four weeks. The calli were then transferred to regeneration medium (ReIII) lacking geneticin. Left panels, the plates were photographed after further incubation for four weeks. Right panels, the percentage of calli that developed shoots was quantified. (A) Infection by an Agrobacterium strain lacking a PolQ gene in the T-DNA. (B) Infection by an Agrobacterium strain containing a PolQ gene in the T-DNA. Data are from Dr. Ayako Nishizawa-Yokoi.