Generation of transgenic chickens and impact of chIFITM1 on hatchability and physiological parameters of hatched chicks. (A) Schema representing the time of transgenesis and challenge experiments. Comparison of hatchability percentage (B), body weight (C), feed intake (D), and water consumption (E) of chIFITM1-expressing transgenic chicken and control chicks (RCASBP(A)-WT and negative control) post-hatching (PH), pre-challenge and post-challenge. Statistical analyses between different inoculated groups were provided within Supplementary Data files 1, 2, 3, and 4. Values of p value < 0.05 were considered statistically significant.