Figure 3.

Immunoblot of p70S6K/p-p70S6K expression indicating a naltrexone/mTOR-dependent interrelation mediated by p70S6K expression and phosphorylation. Protein expression of the total p70 of the S6 kinase (p70S6K) subunit and its phosphorylation fraction p-p70S6K in untreated or naltrexone-treated BV-2^10%FCS cells is shown by Western blot as representative of three individual experiments (n = 3). Quantification analysis indicated statistical significance in the p70S6K expression and phosphorylation ratio change. (*) was set at p ≤ 0.05.