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. 2021 Aug 5;22(16):8410. doi: 10.3390/ijms22168410

Figure 6.

Figure 6

VTN induces NF-κB activation in NaCl-treated BMDMs to upregulate proinflammatory cytokine gene expression. BMDMs were treated with 20 mM NaCl for 24 h and were then treated with 20 µM c(RGDfK), 10 µM SN50, 5 µg/mL of anti-Itg αv antibody, or IgG control for 10 min prior to stimulation with 200 ng/mL of VTN for a further 3 h. (a) Western blotting of NF-κB p65 phosphorylation (p-p65) and total p65 stimulated by VTN for 20~60 min. Representative blots and densitometric analyses from three samples are shown. a p < 0.001versusuntreated cells. b p < 0.005 versus corresponding VTN-treated cells. (b) qPCR analysis of proinflammatory gene expression stimulated by VTN for 3 h. Data were summarized as mean ± SE of three separated experiments. (c) Representative Western blots of p-p65 and total p65 stimulated by VTN for 20 min and densitometric analyses from three samples are shown. c p < 0.002 versus Cont Ab-treated cells. (d) qPCR analysis of proinflammatory gene expression stimulated by VTN for 3 h. Data were summarized as the mean ± SE of three separated experiments. d p < 0.0001 versus Cont Ab-treated cells.