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. 2021 Aug 5;22(16):8410. doi: 10.3390/ijms22168410

Figure 7.

Figure 7

c(RGDfK) suppresses inflammatory responses in dry eyes. The mice received DED induction and c(RGDfK) treatment as described in the legend of Figure 5A. (a,b) Representative images of immunofluorescence staining of NF-κB p-p65 (Ser536) and F4/80 in the conjunctiva after DED induction for 12 days. The p-p65 located in the cell nucleus is confirmed by Hoechst 33258 counterstaining. Asterisks indicate conjunctival epithelium. Arrows indicate p-p65 and F4/80 double-positive cells. There were 30 randomly selected fields in each treatment (n = 6 per group) that were photographed, and the percentage of p-p65 and F4/80 double-positive cells per total F4/80-positive cells was calculated. * p < 0.0006 versus NS mice. # p < 0.0001 versus vehicle group. (c–e) qPCR evaluation of the mRNA levels of proinflammatory cytokines in the conjunctiva after DED induction for 12 days. Values are expressed as mean ± SE. * p < 0.001 versus NS mice. # p < 0.02 versus the vehicle-treated group.