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Sequential FISH identifying each of the ten chromosomes in sorghum. (a) First round of FISH using MCP10 (digoxigenin-red) and MCP9 (biotin-green) probes. (b) Second round of FISH using MCP8 (digoxigenin-red) and MCP7 (biotin-green) probes. (c) Third round of FISH using MCP6 (digoxigenin-red) and MCP5 (biotin-green) probes. Arabic numerals denote the chromosome number in sorghum. Bar = 10 μm.
