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. 2021 Aug 9;22(16):8539. doi: 10.3390/ijms22168539

Figure 3.

Figure 3

Sequential oligo-FISH identifying all 10 chromosomes on the same metaphase plate of T. arundinaceum. (a) Second round of oligo-FISH using MCP5 (biotin-green) and MCP3 (digoxigenin-red) probes. (b) Fourth round of oligo-FISH using MCP9 (biotin-green) and MCP10 (digoxigenin-red) probes. (c) Statistical results of different probes. Arabic numerals denote the chromosome numbers. The dotted box denotes the weak signals of chromosomes 8 and 7. The symbol * indicates chromosomes 5 and 9 were both missing one copy in this particular cell due to the preparation of the slides. The six copies of chromosomes 5 and 9 on metaphase plate are provided in Figure S3. Bar = 10 μm.