Table 1.

Oligonucleotides used to amplify PCR targets for T7E1 assay.

Gene	sgRNA	Step	Direction	Sequence (5′–3′)
USP48	sgRNA1	I PCR	FP	CATTTGGGTGGCTTCCAATA
		I PCR	RP	TAAAACAGGCAGCTGCGTAA
		II PCR	FP	TCCACACCCTCACAAACTGA
		II PCR	RP	TAAAACAGGCAGCTGCGTAA
	sgRNA2	I PCR	FP	CGCTTGTTCAAAACCGATCT
		I PCR	RP	TCTGTTTCCCAAGCCAGAGT
		II PCR	FP	TCCTGTGGTCAACCCAAAAT
		II PCR	RP	TCTGTTTCCCAAGCCAGAGT