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. 2021 Aug 6;22(16):8481. doi: 10.3390/ijms22168481

Figure 1.

Figure 1

YY1 silencing does not affect CRC cellular growth and viability. (A) q-RT-PCR analysis of YY1 expression in the four CRC cell lines (GAPDH used as housekeeping); data expressed as 2−ddCt and compared with SW620 normalized expression. (B) Immunoblot of YY1 (60 KDa) and β-Actin (42 KDa) proteins expression in the four CRC cell lines, densitometry analysis of YY1 expression (normalized to β-Actin). (C) shRNA targeting sequences against luciferase (CTRL) and YY1 (KD-01 and KD-02) transcripts. (D) Immunoblot of YY1 and GAPDH (37 KDa) proteins expression in HCT-116, Caco-2, HT-29 and SW620 clones (CTRL, KD-02, KD-01), densitometry analysis of YY1 expression (as percentage compared to CTRL). (E) MTT-based growth curve of the CTRL, KD-01 and KD-02 HCT-116, Caco-2, HT-29 and SW620 CRC cells (left y-axis: OD at 610 nm over days in culture; right y-axis: fold increase in the OD over the baseline). (F) Doubling time of the CTRL, KD-01 and KD-02 HCT-116, Caco-2, HT-29 and SW620 CRC cells. Values are presented as Mean  ±  SD. * p < 0.05; ** p  <  0.01; **** p  <  0.0001; no asterisk = not significant.