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. 2021 Aug 7;22(16):8515. doi: 10.3390/ijms22168515

Figure 1.

Figure 1

Establishment and identification of global Selenot-KO mice. (A) Global Selenot-KO mice were established via the CRISPR/Cas9 system, where dual sgRNA recognition sites were located on exon 2 of Selenot. (B) The agarose gel electrophoresis image of the PCR products of Selenot from the WT (+/+), heterozygote (+/−) and KO (−/−) mice. (C) Gene sequencing result for smaller bands in the PCR products of Selenot from heterozygous mice (Delete 41 bp on exon 2, resulting in frameshift GGTACCGGCGGGTGT-----------------------------------------ATCCGCATTGAA). (D) Partial gene sequence of Selenot. The gene sequence between the green highlighted parts corresponds to the active center of SELENOT, and the gene sequence between the purple highlighted parts is exon 2, and the yellow highlighted sequences are knocked out. (E) SELENOT protein content in liver and skeletal muscle from the male WT (+/+) and KO (−/−) mice detected by western blot (n = 3). (F) SELENOT protein content in liver and skeletal muscle from the female WT (+/+) and KO (−/−) mice detected by western blot (n = 6).