Figure 4.

The effects of HDAC inhibitor TSA had on BoHV-1 infection-induced DNA damage in A549 cells. (A) A549 cells were infected with BoHV-1 (MOI = 0.1) and treated with either AA or TSA at indicated concentrations, plus a pretreatment for 2 h with indicated inhibitors, respectively. After infection for 48 h, DNA damage in individual cells was determined by using comet assay, the images were acquired under a fluorescence microscope. (B) three hundred cells were randomly selected from each sample for the analysis of TailDNA% with software CASP. Data shown are means ± SD of three independent experiments. Statistical analyses were performed using Student’s t-test (** p < 0.01).