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. 2021 Aug 9;22(16):8579. doi: 10.3390/ijms22168579

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Enrichment of bisecting GlcNAc-bearing proteins with E4-PHA lectin. (A) Illustration of the reaction catalyzed by N-acetylglucosaminyltransferase-III (GnT-III). (B) Experimental design of the present study. Bisecting GlcNAc-bearing glycopeptides from brain membranes of wildtype and Mgat3-deficient mice were enriched with E4-phytohemagglutinin (E4-PHA) lectin and analyzed by liquid chromatography-mass spectrometry (LC-MS). (C) E4-PHA lectin blot of brain membrane proteins from wildtype and Mgat3-decifient (Mgat3^-/-) mice. Western blot with anti-voltage-dependent anion-selective channel 1 (VDAC1) antibody was used as a loading control.