Figure 2.

pEVs isolation methods reported for regenerative approaches. (a) Diagram shows the proportion and the number of reports that used centrifugation at low relative centrifugal force (RCF; lower than 80,000× g), centrifugation at high RCF (higher than 80,000× g), filtration, size exclusion chromatography, other methods or did not report the isolation method used. If two different methods or a combination of methods was used, both groups are represented on the diagram. (b) Diagram represents the platelet source used for pEVs isolation, whether it was platelet rich plasma (PRP), activated PRP or other platelet sources. (c) Diagram shows the storage conditions of the isolated pEVs before use. Temperature conditions at −80 °C, −20 °C, others or not reported conditions are also represented on the figure. A total of 22 articles were reviewed to obtain this data.