Table 2.
Axonal outgrowth and expression of HSP27 in uninjured (control) injured and repaired (experimental), i.e., immediately done or with a delay (7 days) and with a follow-up of 7 days, sciatic nerve and corresponding L4 and L5 (pooled data) dorsal root ganglia (DRG) of healthy Wistar and diabetic Goto-Kakizaki rats.
| Healthy Wistar Rats |
Diabetic Goto-Kakizaki Rats |
||||||
|---|---|---|---|---|---|---|---|
| Treatment | Immediate Repair (7 Days) (n = 8) |
Delayed Repair (7 + 7 Days) (n = 10) |
Immediate Repair (7 Days) (n = 12) |
Delayed Repair (7 + 7 Days) (n = 10) |
p-Values (KW a) |
Fisher’s Method b | |
| Immediate Repair/Delayed Repair | Healthy Rats/ Diabetic Rats |
||||||
| Axonal outgrowth (mm) |
7.0 (5.4–8.2) |
7.9 (6.7–8.9) |
7.3 (7.1–8.2) |
7.8 (5.4–10.9) |
0.43 | NA | NA |
| HSP27 Sciatic nerve Contralateral control (%) |
6.4 (5.9–7.0) |
7.2 (6.5–7.6) |
7.5 (7.0–7.8) |
7.4 (7.2–7.7) |
0.008 | 0.26 | 0.006 |
| HSP27 SNL Experimental (%) |
17.4 (16.2–17.9) |
19.3 (18.8–19.6) |
17.9 (17.6–18.4) |
20.9 (20.4–21.9) |
0.0001 | <0.0001 | 0.0002 |
| HSP27 SNL Exp/control ratio |
2.6 (2.4–3.1) |
2.8 (2.5–2.9) |
2.4 (2.3–2.6) |
2.8 (2.7–2.9) |
0.005 | 0.001 | 0.12 |
| HSP27 SND Experimental (%) |
11.6 (10.2–11.9) |
11.5 (10.5–12.8) |
10.6 (10.3–11.5) |
12.9 (12.4–13.4) |
0.0001 | 0.0009 | 0.0003 |
| HSP27 SND Exp/control ratio |
1.7 (1.5–2.0) |
1.7 (1.4–1.8) |
1.5 (1.3–1.6) |
1.7 (1.6–1.8) |
0.009 | 0.0006 | 0.04 |
| HSP27 DRG Contralateral control (%) |
10.3 (10.0–11.6) |
11.1 (10.2–12.3) |
11.3 (10.8–11.6) |
12.7 (12.2–13.9) |
0.001 | 0.0002 | 0.012 |
| HSP27 DRG Experimental (%) |
21.9 (20.6–22.5) |
21.9 (20.9–23.0) |
21.4 (20.7–21.5) |
22.7 (21.5–26.0) |
0.047 | 0.047 | 0.27 |
| HSP27 DRG Exp/Control ratio |
2.1 (1.8–2.2) |
1.9 (1.7–2.2) |
1.9 (1.8–2.0) |
1.9 (1.6–2.0) |
0.32 | NA | NA |
Values are median (interquartile range; IQR). DRG = dorsal root ganglion. SNL = sciatic nerve close to the site of lesion. SND = the distal sciatic nerve. a Kruskal–Wallis with post hoc Mann–Whitney U-test for individual groups = statistical difference between appropriate groups used for b Fisher’s method for independent samples based on the chi-squared distribution (see methods). NA = not applicable.