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. 2021 Aug 11;22(16):8648. doi: 10.3390/ijms22168648

Figure 8.

Figure 8

Investigation of the NF-κB pathway-dependent inflammatory response in HCPEpiCs using a specific inhibitor. HCPEpiC cells were pretreated with the NF-κB pathway inhibitor BAY 11-7082 (5 µM) for 4 h (gray bar) and then exposed to IVH CSF (red bar) samples (10 v/v %) for 24 h. In the presence of BAY 11-7082, we detected significantly lower HMOX1 (A), IL8 (B), and ICAM1 (C) mRNA levels than samples with IVH CSF alone. The inhibitor alone did not alter these levels compared to the baseline samples. Mean ± SEM values are depicted, n = 3–4/group. The ANOVA test with Bonferroni’s multiple comparison test was used for comparison. Abbreviations: CSF, cerebrospinal fluid; HCPEpiCs, human choroid plexus epithelial cells; HMOX1, heme oxygenase 1; ICAM1, intercellular adhesion molecule 1; IL8, interleukin 8; IVH, intraventricular hemorrhage; mRNA, messenger RNA; and NF-κB, nuclear factor-kappa B.