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A workflow of bioanalysis for therapeutic mAbs by bottom-up, middle-up, and top-down approaches. Reproduced with permission from Todoroki et al. J. Pharm. Biomed. Anal. 2020, 179, 112991 [161]. Abbreviations: HR-MS, high resolution mass spectrometry; LC, liquid chromatography; MS, mass spectroscopy; MS/MS, tandem mass spectroscopy; TOF-MS, time of flight mass spectrometry.
