Figure 1.

Clinical rationale of bacterial antigens and chemotherapeutic drug combination tests. (A) Plated cells were treated with different combinations of bacterial antigens and chemotherapeutic drugs (this group defines the action of chemotherapeutic drugs in the presence of bacterial antigens). (B) Inhibitory drugs were administered in the presence of bacterial antigens after 24 or 72 h of bacterial antigen stimulation (these groups define the effect of chemotherapeutic drugs in the presence of bacterial antigens on existing bacterial antigen-stimulated cancer cells, i.e., in patients with existing oral biofilms). (C) Inhibitory drugs alone were administered after 24 or 72 h of bacterial antigen stimulation (this group defines the effect of chemotherapeutic drugs on existing bacterial antigen-stimulated cancer cells following clinical removal of biofilm with covering antibiotic therapy). (D) Inhibitory drugs were applied in the absence of and without stimulation with bacterial antigens (this group defines a situation where chemotherapeutic drugs are administered to patients who developed oral cancer in the absence of oral biofilm, which is practically impossible in a real-world scenario).