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. 2021 Aug 11;22(16):8641. doi: 10.3390/ijms22168641

Figure 5.

Figure 5

ERK1/2 pathway participation in CA-downregulated MMP1 expression, cell migration and invasion in human HSC3 cells. (A) Human HSC3 cells were treated with various concentrations of CA (0, 2.5, 5 or 10 μM), after which phospho-ERK1/2 and total-ERK1/2 protein levels were measured via immunoblotting analysis. (B,C) Human HSC3 cells were treated with CA (10 μM), siERK2 (50 nM) or CA (10 μM) plus siERK2 (50 nM); and then MMP1 protein level, migration and invasion were further measured. (D,E) Human HSC3 cells were treated with CA (10 μM), U0126 (specific ERK1/2 inhibitor; 50 μM) or CA (10 μM) plus U0126 (50 μM), following which MMP1 protein level, migration and invasion were further measured. GAPDH was used as a protein loading control. Control: untreated cells and scrambled siRNA-transfected cells (for siRNA transfection). *, p < 0.05; **, p < 0.01 versus control (line 1); #, p < 0.05 versus CA (line 2) or siERK2, U0126 (line 3) (Mean ± SE, n = 3). Control: untreated cells. Scale bars: 50 μm.