Figure 6.

Bazedoxifene inhibited SiHa tumor growth in vivo. SiHa cells (1 × 10⁷) were injected subcutaneously into Balb/c nude mice with an equal volume of PBS. When tumors had grown to 150 mm³, mice were administered vehicle or 1, 5, or 10 mg/kg Bazedoxifene daily by oral gavage. (A) Tumor volumes were calculated from caliper measurements. (B) After 14 days of treatment, all mice were sacrificed, and the total mass of each tumor was determined at autopsy (n = 4 mice per treatment group). (C) The tumor masses were excised for comparison between groups. (D) The phosphorylation levels of EMT signaling proteins were determined using Western blot analysis of the harvested tumor tissue. β-actin served as a loading control (**, p < 0.01 ***, p < 0.001 vs. control). B: Bazedoxifene.