Figure 4.

The PROTAC-FCPF degrades dCas9^FCPF, Cas12^FCPF, and Cas13^FCPF. The PROTAC-FCPF induces dCas9^FCPF, Cas12^FCPF, and Cas13^FCPFdegradation. (A) PROTAC-FCPF degrades dCas9^FCPF. HeLa cells expressing dCas9^FCPF were treated with increasing concentrations of PROTAC-FCPF for 8 h. (B, C) PROTAC-FCPF degrades Cas12^FCPF and Cas13^FCPF in (B) concentration-dependent and (C) time-dependent manners. Cells expressing HA-Cas12a^FCPF or HA-Cas13^FCPF were treated with the PROTAC-FCPF as indicated. Immunoblotting was performed. We used HA antibodies to detect HA-tag proteins. We used ACTIN as a loading control. The densitometric analysis of proteins obtained from at least three independent experiments can be found in the Supporting Information (Figure S4), with one of the original pictures as an example.