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. 2021 Jun 7;1(7):998–1013. doi: 10.1021/jacsau.1c00160

Figure 3.

Figure 3

In vivo pharmacokinetic and biodistribution study of DNIC-1 after an oral administration in mice. EPR spectra of (a) stomach (black), stomach after perfusion (blue), and stomach after perfusion and removal of mucus (red), (b) small intestine (black) and small intestine after perfusion (blue), (c) plasma (black), and (d) brain (black) isolated from the mice after an oral administration of DNIC-1. EPR spectra for each organ obtained from the mice without the treatment of DNIC-1 are shown in gray. Time-dependent biodistribution study of DNIC-1 in the (e) stomach and (f) small intestine characterized by EPR spectroscopy, whereas the fitting to pseudo-first-order kinetics is shown in red. The data are the mean values ± SEM (n = 3–5). (g) Pharmacokinetic study of DNIC-1 in the plasma. The data are the mean values ± SEM (n = 3–5) (h) Time-dependent biodistribution study of nitric oxide in the brain. Data show the mean ± SEM from four independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 compared to the concentration of nitric oxide in the brain of mice without the oral administration of DNIC-1. ID g–1 tissue, injected dose per gram of tissue.