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. 2021 Aug 16;22(16):8792. doi: 10.3390/ijms22168792

Figure 4.

Figure 4

SNP rs684232 alters FOXA1 binding. (A) Enrichment of rs684232 region in the FOXA1 ChIP DNA in 22Rv1 cells. IgG and Input as the negative control. Mean ± SD of three technical replicates. *** p < 0.001, two−tailed Student’s t-test. (B) Sanger sequencing chromatography of FOXA1 ChIP DNA for the rs684232 site in 22Rv1 cells. IgG and Input as the negative control. The position of rs684232 is highlighted with a yellow square and arrow. (C) Allele−specific enrichment of rs684232 site in the FOXA1 ChIP DNA determined by AS−qPCR in 22Rv1 cells. Mean ± SD of three technical replicates. ** p < 0.01, two−tailed Student’s t-test.