Figure 1.

Prolonged cortisol exposure induces widespread and cumulative DNA methylation changes. (A). Principal component analysis (PCA) of Illumina EPIC array data showing the progression of cortisol-induced methylomic changes along the cellular lifespan. (B). Venn diagram depicting the number of CpG sites with significant treatment condition-duration interaction and methylation differences between cortisol and vehicle groups at middle or late passage. (C). Boxplots comparing the magnitude of cortisol-induced methylation changes (hyper- or hypo-methylation) at middle and late passage. Each datapoint represents a significantly changing CpG and methylation changes.