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. 2021 Jul 27;7(3):45. doi: 10.3390/ncrna7030045

Figure 1.

Figure 1

Relative expression of miR-203 performed by miRNA-specific quantitative RT-PCR. (A) miR-203 expression in breast cancer cell lines and in human mammary epithelial (HMLE) cells induced to undergo epithelial–mesenchymal transition (EMT) in response to Twist overexpression. (B) miR-203 expression in indicated cell lines transduced with a retrovirus vector to stably overexpress miR-203. (C) miR-203 expression in indicated cell lines exposed to microRNA mimic-loaded nanoliposomes at a concentration of 85 μM for 48 h. Data are presented as the mean and SEM of three independent replicates, two-tailed Student’s t-test. (D) miR-203 expression in indicated cell lines for the indicated number of days post-exposure to microRNA-203 mimic-loaded nanoliposomes. Expression is presented relative to control cells exposed to scramble-loaded nanoliposomes. Small nucleolar RNA U6 was used as a normalization control. Data are presented as the mean and SEM of three independent replicates, one-way analysis of variance (ANOVA) with Dunnet’s correction for multiple hypothesis testing. * p < 0.05, ** p < 0.01, *** p < 0.001.