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. 2021 Jul 27;7(3):45. doi: 10.3390/ncrna7030045

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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mRNA expression was measured by Nanostring PlexSet analysis and qRT-PCR. (a) Counts were normalized to three control genes and represented as z-scores within a hierarchical cluster. (b,c) Expression of indicated genes for cells with constitutive (b) or nanoliposomal-mediated (c) expression of miR-203 was determined using qRT-PCR. Mean and SEM of three biological replicates measured in technical quadruplicate are shown. Expression determined relative to vector or NL-scr control and normalized to GAPDH. p-value calculated using Student’s two-tailed t-test. * p < 0.05, ** p < 0.01.

mRNA expression was measured by Nanostring PlexSet analysis and qRT-PCR. (a) Counts were normalized to three control genes and represented as z-scores within a hierarchical cluster. (b,c) Expression of indicated genes for cells with constitutive (b) or nanoliposomal-mediated (c) expression of miR-203 was determined using qRT-PCR. Mean and SEM of three biological replicates measured in technical quadruplicate are shown. Expression determined relative to vector or NL-scr control and normalized to GAPDH. p-value calculated using Student’s two-tailed t-test. * p < 0.05, ** p < 0.01.