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. 2021 Aug 13;22(16):8715. doi: 10.3390/ijms22168715

Figure 2.

Figure 2

Effects of regular exercise on iron load in cortex and muscle tissues in 5xFAD mouse model. Total iron content, ferritin, and HO-1 level in cortex (A–D) and muscle samples (E–G) of WT and 5xFAD mice. Total iron content in cortex (A) and muscle (E) was measured by ICP-MS. mRNA expression of ferritin and HO-1 in cortex (B) and muscle (F) was measured by qPCR. (C) Representative images of ferritin levels in cortex of WT-SED, WT-EXE, 5xFAD-SED, and 5xFAD-EXE mice. Scale bar 200 μm. (D) Percentage of immunoreactive area was quantified to measure ferritin level in cortex. (G) Representative Ponceau S staining and Western blot of ferritin in muscle samples and the analysis of band intensities normalized to the total proteins. All data are presented as mean ± SEM. n = 8/group. «#» exercise effect: *** p < 0.001, ## p < 0.01, # p < 0.05. General genotype/exercise effect among all groups is presented as a line with «#» sign on top, exercise effect in 5xFAD mice only is presented as a bracket with «#» sign on top.