Skip to main content
. 2021 Aug 14;22(16):8746. doi: 10.3390/ijms22168746

Figure 8.

Figure 8

After IPEC-J2 cells were treated for 48 h with 1000 U/mL TNFα, a medium exchange was carried out and cells were either further incubated with the cytokine (TNFα without recovery; w/o R) or incubated with normal medium without TNFα (TNFα with recovery; w/R) for another 48 h. (A) TEER values of groups treated with TNFα removal were compared to cells treated without removal of the cytokine, respectively (one-way ANOVA, ** p < 0.01; n = 7–13). (B) Representative Western blot bands and (C) Densitometry of IPEC-J2 cells with or without recovery after 48 h incubation with TNFα revealed an increase in claudin-1 and occludin after removal of the cytokine, while claudin-3 expression did not show significant changes. The expression level of TNFR-1 also did not seem to be affected by TNFα removal. The shown data are presented as mean ± SEM (one-way ANOVA, * p < 0.05, ** p < 0.01, *** p < 0.001; n = 3).